StepCore OperationSafety/Quality AssuranceKey Technical PointsFinal Core Value
01
Sample Collection
Collect a small biological sample from the racing pigeon, usually 2-3 feathers with intact follicles (base of the shaft), or collect a micro drop of blood on specialized filter paper.
Minimally invasive sampling method ensures the safety and comfort of the racing pigeon throughout the sampling process.
Non-invasive sampling, no professional blood collection required, can be done independently by ordinary users.
Minimal sampling threshold, minimizing stress and physical harm to the racing pigeon.
02
Sample Submission
Place the collected sample securely in sterile packaging, and ship the package to the designated address following the simple transport instructions provided by the official.
Sterile packaging prevents contamination and degradation, and standardized transport procedures ensure sample integrity throughout.
Standardized packaging and transport guidelines, compatible with regular courier logistics, no special cold chain transport required.
Ensure sample DNA integrity, laying the core foundation for the reliability of subsequent test results.
03
DNA Extraction
After the sample arrives at the laboratory, a certified professional laboratory team separates and extracts high-purity DNA from the feather/blood sample.
Use certified reagents and industry-validated standardized experimental protocols, fully compliant with laboratory biosafety regulations.
Optimized DNA extraction protocol maximizes yield and purity of target DNA, removing impurity interference.
Obtain high-quality DNA templates, ensuring accuracy and stability in subsequent amplification and detection steps.
04
PCR Amplification
Using polymerase chain reaction (PCR) technology to specifically amplify target genes on the sex chromosomes of racing pigeons.
Standardized PCR amplification system, strictly controlled experimental environment to avoid non-specific amplification and sample cross-contamination.
Targeted amplification of the CHD gene (chromodomain helicase DNA binding gene) on the Z/W sex chromosomes of racing pigeons, generating billions of target DNA copies.
Amplifying trace target DNA to a stably detectable level is the core technical step for achieving high-precision identification.
05
Result Analysis
Professional technicians use gel electrophoresis or real-time quantitative PCR (RT-PCR) to precisely analyze and interpret the amplified DNA fragments.
Dual technical path cross-validation to avoid misinterpretation, and double-person review throughout to ensure accuracy.
Diagnosis is made by the presence and size of DNA fragments: male pigeon (ZZ) shows only 1 specific DNA band; female pigeon (ZW) shows 2 specific DNA bands of different lengths.
Achieves high-precision determination, with single sample test accuracy up to 99.9%.
06
Report Delivery
Deliver an officially certified and easy-to-understand formal test report to the user, supporting both email and printed paper delivery.
The report includes a unique digital certification, making test results traceable and verifiable, with industry recognition.
Standardized report template clearly presenting test results, technical explanations, accuracy assurance, and sampling precautions.
Results can be delivered within 2-15 business days after the laboratory receives the sample, providing users with fast, authoritative, and reliable racing pigeon identification conclusions.